电刺激束缚小鼠应激诱发胃溃疡及其机制的初步探讨

用电刺激躯体束缚的口唇部建立应生胃溃疡模型，以阿尔新兰－ＭｇＣｌ２洗脱法测定胃壁粘液量，研究安定、苯巴比妥钠、可的松、酚妥拉明、异丙上腺素、阿托品和乙醇对溃疡发生的影响，结果表明，安定、苯巴比妥负伤 异丙肾上腺素显著抑制溃疡发生；阿托品和乙醉可明显使溃疡加剧；酚托拉明和可的松对溃疡没有影响，模型动物胃壁粘液量与对照组比较以及所有药物对模型动物胃壁粘液量却没有明显变化，提示，电刺激束缚应激胃溃疡的发     

日本沼虾精荚结构的研究

应用光镜和透射电镜对日本沼虾精荚的结构研究表明：单个精荚由精子团，粘液团和非细胞的包膜三部分组成。包膜具单层结构，主要由细小的相交纤丝所组成的基质构成。在超微结构上，包膜可再分成内，外侧二个区域。包膜几乎完全包被精子团和粘液团。精子团由精子和非细胞基质构成，基质包括沟网，球状体和丝线网三部分，它们分别和精子间在不同的部位连结。     

牦母牛发情期阴道粘液pH值、粘液结晶、细胞学变化规律与受胎率关系的研究

对１９头健康牦母牛在发情期中阴道粘液的变化情况进行了研究，结果表明，在友情盛期，牦母牛阴道粘液ｐＨ值为７；１２±０．３１，阴道分泌物抹片镜检可见大量无核上皮细胞，少量有核上皮细胞，很少见白细胞，并有５２．６％牛只出现不同形状的粘液结晶；在发情末期，阴道粘液ｐＨ值为６．９７±０．２７，镜检可见上皮细胞减少，白细胞增多，粘液结晶出现率明显减少仅占３１．２％；受试１９头牦母牛中，受孕数１３头，受胎率为６８．４％，其中，１０头出现粘液结晶的发情牦母牛全部受孕（１００％），９头未发现粘液结晶的牛只仅受孕３头（３３．３％），二者差异极显著（Ｐ＜０．０１），由此看出，牦母牛在友情期中阴道粘液结晶的出现率与会胎率成正相关。该研究结果为准确判定牦母牛发情期，指导过时输精及提高杂交受胎率，提供了简便易行的方法和基础参考数据。     

高能μ子在铁和铅中的能量损失研究

研究了能量从１０ＧｅＶ到１０００ＧｅＶ的μ子在称之为ＬＡＡ的探测器装置的铅量能器和铁过滤器中的能量损失，得出了高能μ子在铅和铁中的能量损失率随μ子能量的变化．     

The trefoil factor family – small peptides with multiple functionalities

The trefoil factor family (TFF) comprises a group of small peptides which are highly expressed in tissues containing mucus-producing cells – especially in the mucosa lining the gastrointestinal tract. The peptides seem crucial for epithelial restitution and may work via other pathways than the conventional factors involved in restitution. In vitro studies have shown that the TFFs promote restitution using multiple mechanisms. The peptides also have other functionalities including interactions with the immune system. Moreover, therapeutic effects of the TFFs have been shown in several animal models of gastrointestinal damage. Still it is not clear which of their in vitro properties are involved in the in vivo mode of action. This review describes the TFF family with emphasis on their biological properties and involvement in mucosal protection and repair. Received 10 October 2008; received after revision 07 November 2008; accepted 10 November 2008     

改良式腹腔镜辅助下阴式子宫切除术5例

目的介绍改良式腹腔镜辅助下阴式子宫切除术。方法在LAVH-I型的基础上结合部分Ⅲ型,即在镜下离断双侧圆韧带、输卵管、卵巢固有韧带（或卵巢悬韧带）,打通阴道后穹窿,并离断双侧骶韧带;经阴道处理主韧带及子宫血管。结果5例患者全部成功实施改良式腹腔镜辅助下阴式子宫切除,手术时间90～150 min,平均110 min,术中出血80～250 mL,平均165 mL,无1例大出血、副损伤及术后病率。结论改良式腹腔镜辅助下阴式子宫切除术既克服了开腹手术和阴式手术的缺陷,降低手术难度,缩短手术时间,减少术中出血,特别适合初学者和基层医院开展,是子宫切除方式的又一术式选择。     

The trefoil protein TFF1 is bound to MUC5AC in humangastric mucosa

The trefoil protein TFF1 is expressed principally in the superficial cells of the gastric mucosa. It is a small protein and forms homo- and hetero-dimers via a disulphide bond through Cys58 which is located three amino acids from the C terminus. TFF1 is co-expressed with the secreted mucin MUC5AC in superficial cells of the gastric mucosa suggesting that it could be involved in the packaging or function of gastric mucus. We have previously shown that TFF1 co-sediments with mucin glycoproteins on caesium chloride gradients. To extend this observation we have now used gel filtration under physiological conditions, immunoprecipitation and Western transfer analysis to characterise the interaction of TFF1 with gastric mucin glycoproteins. We show that TFF1 co-elutes with MUC5AC but not MUC6 on gel filtration and that immunoprecipitation and Western transfer analysis confirms that TFF1 interacts with MUC5AC. We also demonstrate that the TFF1 dimer is the predominant molecular form bound to MUC5AC. Salt and chelators of divalent cations such as EDTA and EGTA disrupted the TFF1- MUC5AC interaction and increased the degradation of MUC5AC, whereas calcium increased the amount of TFF1 bound to MUC5AC. These data support the contention that TFF1 is pivotal in the packaging and function of human gastric mucusa.Received 24 March 2004; received after revision 14 May 2004; accepted 7 June 2004     

脑室注射GABA对小鼠实验性胃溃疡及其粘液屏障的影响

报道小白鼠脑室注射ＧＡＢＡ（４ｍｌ）对无水乙醇（０．ｌｍｌ灌胃）、盐酸（０．２Ｎ，０．２ｍｌ灌胃）、消炎痛（３２ｍｇ·ｋｇ－１肌肉注射）和冷束缚应激（１℃）四种不同实验性胃溃疡及其粘液屏障的影响。实验结果表明：乙醇、盐酸和冷束缚诱导胃溃疡时，胃壁粘液量都显著减少，而消炎痛则无变化。脑室注射ＧＡＢＡ能加剧乙醇诱导型、消炎痛诱导型这两种胃溃疡，但对胃壁粘液量无明显影响。而脑室注射ＧＡＢＡ能抑制盐酸诱导型、冷束缚应激型胃溃疡，并使固壁粘液明显升高。本文对中枢ＧＡＢＡ能机制参与胃溃疡及胃粘液调节作了进一步探讨。     

拮抗菌对病原性溶藻弧菌粘附大黄鱼鳃粘液的影响

用3H-TdR同位素标记方法研究了分离自患病大黄鱼的溶藻弧菌和健康大黄鱼的7株拮抗菌对大黄鱼鳃粘液的粘附动力学以及拮抗菌对溶藻弧菌粘附的影响,试验结果表明:溶藻弧菌和7株拮抗菌对大黄鱼鳃粘液的粘附都属于饱和粘附;溶藻弧菌对大黄鱼鳃粘液的最大粘附量为4.1×105cells/well,7株拮抗菌的最大粘附量和亲和指数都高于溶藻弧菌;各菌株的分离常数都高于自身的最大粘附量,总体上呈现最大粘附量高的菌株其分离常数也大的趋势.7株拮抗菌在置换条件下都能显著降低溶藻弧菌对鳃粘液的粘附量,在竞争条件下有5株能够显著降低溶藻弧菌的粘附量,在排斥条件下仅有3株能够显著降低溶藻弧菌的粘附.结果表明:病原性溶藻弧菌对大黄鱼鳃粘液有较强的粘附作用;7株拮抗菌能够抑制溶藻弧菌的粘附,其中置换作用效果最好,排斥作用效果最差;拮抗菌和病原菌的粘附动力学特征可在一定程度上反映它们的相互作用效果,但是仅仅根据各菌株自身的粘附动力学特性无法推断其对病原菌粘附的抑制能力.     

确定小鼠动情周期的三种方法

目的为选择确定小鼠动情期的最准确和方便的方法,取成熟雌性昆明小鼠,每日晨取阴道脱落细胞及宫颈黏液做涂片,置显微镜下观察,分别用宫颈黏液结晶法、阴道脱落细胞学检查法、阴道脱落角化细胞计数法三种不同方法来确定小鼠动情期。结果显示:三种方法均可以确定小鼠动情期,但三种方法比较,宫颈黏液结晶法特异性差;阴道脱落细胞学方法较为准确,但有假阴性现象;角化细胞计数法直观方便,结果最为客观准确。